Evaluation of the Carcinogenicity of Aminofluorenols by Implantation into the Bladder of the Mouse.

To test the hypothesis that o-hydroxy amines are proximate agents in chemical carcinogenesis paraffin pellets containing the hydrochlorides of 2-amino-l-fluorenol, 2-amino-3-fluorenol, 2-amino-5-fluorenol, 2-amino-7-fluorenol, 2-fluorenamine, and 1amino-2-naphthol were implanted into the urinary bladders of Swiss female mice. Paraffin pellets alone were implanted as controls. Histological examination of the bladders 40 weeks after implantation showed no evidence of carcinoma. Inflammatory changes, squamous metaplasia, and epithelial hyperplasia were noted with equal fre quency in the control and experimental animals. The stability of implanted 2-aminol-fluorenol-1-C14 hydrochloride and l-amino-2-naphthol-H3 hydrochloride and the elution of these compounds from the pellets were examined by radioactive tracer methods. Implanted 2-amino-l-fluorenol-l-C14 hydrochloride was stable, whereas l-amino-2-naphthol-H3 hydrochloride decomposed soon after implantation. The severe